St. Mark's Church-in-the-Bowery, ca. 1979.

The land on which this 1799 church sits was once farmland owned by the Dutch West India Company. In 1651, Peter Stuyvesant, Director General of New Netherland, purchased the farm or "bouwerie," and constructed a chapel. When Stuyvesant died in 1672, he was buried under the chapel (he remains are still interred here). A descendant sold the old chapel and its land to the Episcopal Church under the condition that a new structure be erected. The cornerstone of the late Georgian style church was laid in 1795 and the fieldstone building was consecrated in 1799. Its architect, John McComb Jr., was the designer of a number of iconic late 18th and early 19th century landmarks, including Gracie Mansion, City Hall, Hamilton Grange, and Castle Clinton. At the turn of the 19th century Alexander Hamilton assisting in the legal efforts to recognize St. Mark's Church as the first American Episcopal parish autonomous of the 1697-founded Trinity Church.

In 1828 a Greek Revival steeple was added to the building, with renovated columns, a new parish house, a Sunday school, and a cast- and wrought iron fence all completed in the 1830s. Later, a cast iron portico (attributed to cast iron innovator James Bogardus) was constructed.

While the parish had long been engaged in charity work, the 20th century saw the community expand their social justice and cultural efforts, hosting multicultural religious leaders, dance, poetry, and art events, and work training programs. Following a devastating fire in 1978, the community and local preservationists banned together to form the Citizens to Save St Mark's. Through advocacy, financial support, and the physical labors of the Preservation Youth Project, the building restoration was completed in 1986.